LCMS:Acori-graminei
From Metabolomics.JP
(Difference between revisions)
| Line 13: | Line 13: | ||
|PCapillary =200 | |PCapillary =200 | ||
|PGas =1.5 | |PGas =1.5 | ||
| + | }} | ||
| + | |||
| + | {{CrudeDrug/LCMS/Neg | ||
| + | |NInstrument =Shimadzu LC-IT-TOF MS ESI | ||
| + | |NColumn =Waters Atlantis T3 (2.1 mm x 150 mm) | ||
| + | |NTemperature=40 | ||
| + | |NSolventA =5 mM Ammonium acetate solution | ||
| + | |NSolventB =acetonitrile | ||
| + | |NGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | ||
| + | |NVoltage =?3.5 | ||
| + | |NCapillary =200 | ||
| + | |NGas =1.5 | ||
}} | }} | ||
Revision as of 14:49, 10 February 2011
TIC
Analytical conditions (Positive mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) |
| Column temperature | 40 °C |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | 4.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
Analytical conditions (Negative mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 150 mm) |
| Column temperature | 40 °C |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | ?3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
