LCMS:Yokukansan

Revision as of 18:38, 10 February 2011

Article Title: “Ameliorative effects of Yokukansan on learning and memory deficits in olfactory bulbectomized mice.”
Authors: Yamada et.al

LC-MS TIC of extracts

LC-MS TIC of standard compounds

Instrument	Shimadzu LC-IT-TOF MS ESI (Positive ion mode)
Column	Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
Column Temperature	40℃
Solvent A	5 mM Ammonium acetate solution
Solvent B	acetonitrile
Gradient	10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage	4.5 kV
Capillary temperature	200 °C
Nebulizer gas	1.5 l/min

@@ Line 8: / Line 8: @@
 [[Image:Yokukann2.jpg|thumb|700px|center|LC-MS TIC of standard compounds]]
-{{{CrudeDrug/LCMS/Pos
+==Analytical conditions==
-|PInstrument =Shimadzu LC-IT-TOF MS ESI
+{| class="wikitable" style="width:80%"
-|PColumn     =Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
+|-
-|PTemperature=40
+| Instrument || Shimadzu LC-IT-TOF MS ESI (Positive ion mode)
-|PSolventA   =5 mM Ammonium acetate solution
+|-
-|PSolventB   =acetonitrile
+| Column || Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
-|PGradient   =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
+|-
-|PVoltage    =4.5
+| Column Temperature|| 40℃
-|PCapillary  =200
+|-
-|PGas        =1.5
+| Solvent A || 5 mM Ammonium acetate solution
-}}
+|-
+| Solvent B || acetonitrile
+|-
+| Gradient || 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
+|-
+| Source voltage ||  4.5 kV
+|-
+| Capillary temperature || 200 °C
+|-
+| Nebulizer gas || 1.5 l/min
+|}
 ==LC-MS raw data==