LCMS:Albizzae Cortex
From Metabolomics.JP
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|PColumn =Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) | |PColumn =Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) | ||
|PTemperature=40 | |PTemperature=40 | ||
| − | | | + | |PSolventA =5 mM Ammonium acetate solution |
| − | | | + | |PSolventB =acetonitrile |
|PGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | |PGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | ||
|PVoltage =4.5 | |PVoltage =4.5 | ||
| Line 19: | Line 19: | ||
|NColumn =Waters Atlantis T3 (2.1 mm x 150 mm) | |NColumn =Waters Atlantis T3 (2.1 mm x 150 mm) | ||
|NTemperature=40 | |NTemperature=40 | ||
| − | | | + | |NSolventA =5 mM Ammonium acetate solution |
| − | | | + | |NSolventB =acetonitrile |
|NGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | |NGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | ||
|NVoltage =-3.5 | |NVoltage =-3.5 | ||
Revision as of 13:25, 10 February 2011
TIC
Analytical conditions (Positive mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) |
| Column temperature | 40 °C |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | 4.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
Analytical conditions (Negative mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 150 mm) |
| Column temperature | 40 °C |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | -3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
